Publications
2024
Farrants, H., Shuai, Y., Lemon, W. C., Monroy Hernandez, C., Zhang, D., Yang, S., Patel, R., Qiao, G., Frei, M. S., Plutkis, S. E., Grimm, J. B., Hanson, T. L., Tomaska, F., Turner, G. C., Stringer, C., Keller, P. J., Beyene, A. G., Chen, Y., Liang, Y., Lavis, L. D., Schreiter, E. R., "A modular chemigenetic calcium indicator for multiplexed in vivo functional imaging" Nat Methods 2024. external page doi: 10.1038/s41592-024-02411-6
Frei, M. S., Sanchez, S. A., Liu, L., Schneider, F., Wang, Z., Hakozaki, H., Li, Y., Lyons, A. C., Rohm, T. V., Olefsky, J. M., Shi, L., Schöneberg, J., Fraser, S. E., Mehta, S., Wang, Y., Zhang, J., "Far-red chemigenetic biosensors for multi-dimensional and super-resolved kinase activity imaging" bioRxiv 2024. external page doi: 10.1101/2024.02.10.579766
Frei, M. S., Mehta, S., Zhang, J., "Next-generation genetically encoded fluorescent biosensors illuminate cell signaling and metabolism" Annu. Rev. Biophys. 2024, 53, 275-297. external page doi: 10.1146/annurev-biophys-030722-021359
2023
Hellweg, L., Edenhofer, A., Barck, L., Huppertz, M.-C., Frei, M. S., Tarnawski, M., Bergner, A., Koch, B., Johnsson, K., Hiblot, J., “A general method for the development of multicolor biosensors with large dynamic ranges” Nat. Chem. Biol. 2023, 19, 1147-1157. external page doi: 10.1038/s41589-023-01350-1
Xue, L., Schnacke, P., Frei, M. S., Koch, B., Hiblot, J., Wombacher, R., Fabritz, S., Johnsson, K. “Probing coenzyme A homeostasis with semisynthetic biosensors” Nat. Chem. Biol. 2023, 19, 346–355. external page doi: 10.1038/s41589-022-01172-7
Kompa, J., Bruins, J., Glogger, M., Wilhelm, J., Frei, M. S., Tarnawski, M., D’Este, E., Heilemann, M., Hiblot, J., Johnsson, K. “Exchangeable HaloTag ligands for super-resolution fluorescence microscopy” J. Am. Chem. Soc. 2023, 145, 3075–3083. external page doi: 10.1021/jacs.2c11969
2022
Frei, M. S.*, Koch, B., Hiblot, J., Johnsson, K.* “Live-cell fluorescence lifetime multiplexing using synthetic fluorescent probes” ACS Chem. Biol. 2022, 17, 1321–1327. external page doi: 10.1021/acschembio.2c00041 * Co-corresponding
Frei, M. S., Tarnawski, M., Roberti, M. J., Koch, B., Hiblot, J., Johnsson, K. “Engineered HaloTag variants for fluorescence lifetime multiplexing” Nat. Methods 2022, 19, 65–70. external page doi: 10.1038/s41592-021-01341-x
2019
Frei, M. S., Hoess, P., Lampe, M., Nijmeijer, B., Kueblbeck, M., Ellenberg, J., Wadepohl, H., Ries, J., Pitsch, S., Reymond, L., Johnsson, K. “Photoactivation of silicon rhodamines via a light-induced protonation“ Nat. Commun. 2019, 10, 4580. external page doi: 10.1038/s41467-019-12480-3
Wang, L., Frei, M. S., Salim, A., Johnsson, K. “Small-Molecule Fluorescent Probes for Live-Cell Super-Resolution Microscopy” J. Am. Chem. Soc. 2019, 141, 2770–2781. external page doi: 10.1021/jacs.8b11134
2017
Schwertz, G., Frei, M. S., Witschel, M. C., Rottmann, M., Leartsakulpanich, U., Chitnumsub, P., Jaruwat, A., Ittarat, W., Schäfer, A., Aponte, R. A., Trapp, N., Mark, K., Chaiyen, P., Diederich, F. “Conformational Aspects in the Design of Inhibitors for Serine Hydroxymethyltransferase (SHMT): Biphenyl, Aryl Sulfonamide, and Aryl Sulfone Motifs” Chem. Eur. J. 2017, 23, 14345–14357. external page doi: 10.1002/chem.201703244
2015
Frei, M. S., Bilyard, M. K., Alanine, T. A., Galloway, W. R. J. D., Stokes, J. E., Spring, D. R. “Studies towards the synthesis of indolizin-5(3H)-one derivatives and related 6,5-azabicyclic scaffolds by ring-closing metathesis” Bioorg. Med. Chem. 2015, 23, 2666–2679. external page doi: 10.1016/j.bmc.2014.11.037